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The traces are nearly indistinguishable due to the tight uniformity. This graph shows the temperature measured by probes in 15 wells across a sample block. Superior uniformity with rapid arrival at target temperature. Now you can tailor your runs around your schedule instead of tailoring your schedule around your runs. Run times can be dramatically shortened - to less than 30 min - while still producing accurate quantitative results. The CFX96 Touch system produces high average ramp rates and tight uniformity during ramping to yield fast time to target temperature and faster protocol run times. Maximum ramp rate is less important because it can fluctuate significantly during the ramp. Rapid Arrival at Target Temperature A key component of overall protocol run time is the time required to reach target temperature, which is determined by the average ramp rate and the time needed for the sample block to reach thermal uniformity. To obtain reliable, consistent results, all sample wells must maintain proper temperature throughout each incubation step. Superior Uniformity Precision of the temperature steps is critical for the rate and efficiency of PCR. Reduced-mass sample block - fast ramping and settling produce the shortest time to target temperature available in a thermal cycler. Thermal gradient feature - identifies optimal annealing temperature in a single run. Protocol autowriter - generates an optimal protocol based on your polymerase, primers, and product length. Bio-Rad engineers have developed a complete thermal cycling system that will speed every step of the PCR process to give you what's really important - a shorter time from setup to results. Many factors contribute to the overall time it takes to get PCR results: the time to design, program, and optimize a protocol, the time to run the protocol, and the time to analyze the results. If you're like most researchers, you want to get results faster. The optical filter sets are designed to maximize fluorescence detection for specific dyes in specific channels.Īt every position and with every scan, the optics shuttle is reproducibly centered above each well, so the light path is always fixed and optimal, and there is no need to sacrifice data collection in one of the channels to normalize to a passive reference. The optical system automatically collects data from all wells during data acquisition, so you can enter or edit well information on your own schedule. Scanning just above the sample plate, the optics shuttle individually illuminates and detects fluorescence from each well with high sensitivity and no cross talk.
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